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m although the Zn enzyme has the bottom. Identical developments inside the variety of the catalytic constants have been documented Beforehand for your Co, Zn, Mn and Fe reconstitutions of apo-NagA from E. coli
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cat vs. pH profile, just one ionization is observed for either Zn- or Cd-NagA. This ionization must stand for the protonation in the metallic-bound hydroxide that is definitely used for substrate hydrolysis link naga26 or, alternatively, the overall foundation that's used to deprotonate the metal-bound water molecule previous to substrate hydrolysis. The p
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